Recombinant human Prolactin produced in
E. coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23 kDa. The Prolactin is purified by proprietary chromatographic techniques.
Synonyms: Ma mMotropin, Luteotropic hormone, Luteotropin, PRL.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 10 mM sodium phosphate buffer pH 8 and 50 mM Nacl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ile-Cys.
Uniprot ACC number: P01236
Transportation method: Shipped at room temperature.
Stability: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prolactin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lympH oma, Nb2-11 was found to be < 0.065 ng/ml corresponding to a Specific Activity of 15, 385,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com