Recombinant human Pro-IGF2 produced in HEK cells is a glycosylated monomer, contains 157 a.a.. (24-180) having a total molecular weight of 25 kDa. The Pro-IGF2 contains a C-terminal propeptide (E peptide) Arg92 to Lys180 and is purified by proprietary chromatographic techniques.
Synonyms: Pro-Insulin Like Growth Factor-2, pro-IGF2.
Formulation: The Pro-IGF2 was Lyophilized in 50 mM Sodium Acetate pH 4.5 and 350 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as obsereved by SDS-PAGE.
Source: HEK.
Uniprot ACC number:Transportation method: Shipped at room temperature.
Stability: Lyophilized Pro-IGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Pro-IGF2 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line) and is typically 2-8 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized Pro-IGF2 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Safety data sheet: Inquire at info@3hbiomedical.com