Recombinant human proBDNF produced in E. coli is a single, non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 229 amino acids and having a molecular mass of 52 kDa. The proBDNF is purified by proprietary chromatographic techniques. Synonyms: proBDNF, Precursor Form Brain-derived Neurotrophic Factor. Formulation: proBDNF was lyophilized from a concentrated (0.5 mg/ml) solution in 20 mM PB, pH 8.0 and 500 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Met-Lys.
Uniprot ACC number: P23560
Transportation method: Shipped at room temperature.
Stability: Lyophilized proBDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution proBDNF should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized proBDNF in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com