Recombinant human Platelet-Derived Growth Factor BB is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.3 kDa. PDGF-BB is purified by proprietary chromatographic techniques.
Synonyms: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.
Uniprot ACC number: P01127
Transportation method: Shipped at room temperature.
Stability: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Platelet-derived growth factor mediates survival of leukemic large granular Lymphocytes via an autocrine regulatory pathway. Publication:Published online before print October 30, 2009, doi: 10.1182/blood-2009-06-223719 Blood January 7, 2010 vol.115 no.1 51-60 . Link:http://bloodjournal. hematologylibrary. org/content/115/1/51. full2. Title: attenuates skeletal muscle dystropH y in mdx mice. Publication:Published online before print March 16, 2009, doi: 10.109 6/fj.09-129833 August 2009 The FASEB Journal vol.23 no.8 2539-2548. Link:http://www. fasebj. org/content/23/8/2539. full 3. Title:Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing. Publication: Cell Transplantation, Vol.19, pp.1635–1644, 2010 0963-6897/10 $900 + .00 Printed in the USa.a.ll rights reserved. DOI: 10.3727/096368910X516637 Copyright ? 2010 Cognizant Co mM. Corp. Link:http://docserver. ingentaconnect. com/deliver/connect/cog/09636897/v19n12/s11. pdf?expires=1370351771&id=74450861&titleid=5476&accname=Guest+User&checksum=14BACCDE2ACF33
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