Recombinant human Platelet-derived Growth Factor AB is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids (and an N-terminal Met) consisting of 14.3 kDa Alpha-chain and 12.1 beta-chain having a total molecular mass of 26.4 kDa. PDGF-AB is purified by proprietary chromatographic techniques.
Synonyms: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB.
Formulation: lyophilized from 10 mM AcOH (Acetic Acid).
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE analysis.
Source:Escherichia coli. Amino acid sequence: Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT. Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT.
Uniprot ACC number:Transportation method: Shipped at room temperature.
Stability: Lyophilized Platelet-derived Growth Factor AB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AB should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent proliferation of mouse 3T3 indicator cells, is 1.4-2.1 ng/ml
. This corresponds to a specific activity of 7.1x 105 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AB in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com