Recombinant human PLGF1 produced in
E. coli is a single, non-glycosylated, polypeptide chain (a.a. 19-131) containing 123 amino acids including a 10 a.a. N-terminal His-Tag. The total molecular mass is 13.8 kDa (calculated).
Synonyms: PIGF, PGF, PLGF-1.
Formulation: PLGF1 filtered (0.4 µm) and lyophilized from 0.5 mg/ml in PBS and 5% w/v trehalose, pH 7.4.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHAS LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECR.
Uniprot ACC number: P49763
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. PLGF1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com