Recombinant human Placental Growth Factor-2 produced in
E. coli is a non-glycosylated, disulfide-linked homodimer consisting of 2x152 amino acid polypeptide chains, having a total molecular mass of approximately 34.6 kDa. PLGF2 is purified by proprietary chromatographic techniques.
Synonyms: PIGF, PGF, PlGF-2, PLGF-2.
Formulation: lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4 and 0.02 % Tween-20.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR.
Uniprot ACC number: P49763
Transportation method: Shipped at room temperature.
Stability: Lyophilized PLGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Placental Growth Factor-2 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 5.0-50 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized Placental Growth Factor-2 in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com