Recombinant human Placenta Growth Factor-1 produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of approximately 34 kDa. The PLGF-1 is purified by proprietary chromatographic techniques.
Synonyms: PIGF, PGF, PLGF-1.
Formulation: lyophilized from a concentrated (1mg/ml) solution containing 50 mM acetic acid.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Insect Cells.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.
Uniprot ACC number: P49763
Transportation method: Shipped at room temperature.
Stability: Lyophilized Placenta Growth Factor 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by its ability to bind to i mMobilized rh-sFlt-1 in a functional ELISA. PlGF-1 human Recombinant can bind to i mMobilized rh-sFlt-1 (100ng/well) with a linear range at 0.5-10 ng/ml, corresponding to a Specific Activity of 1x105 -2x106 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 0.1M acetic acid not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com