Recombinant Human OCIF produced in
E. coli cells is a single, non-glycosylated, polypeptide chain containing amino acids 201-401 and having a molecular mass of 31 kDa which includes a 4 kDa His-Tag. The OPG is purified by proprietary chromatographic techniques.
Synonyms: TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, TR1, MGC29565.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1 mM DTT.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 80% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Uniprot ACC number: O00300
Transportation method: Shipped at room temperature.
Stability: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Applications: 1. Positive control for Western blot.2. Antibody production.3. Protein assay.
Solubility: It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com