Recombinant OPG produced in yeast contains 2x412 amino acid residues, including 180 residues from mature OPG (a.a. 22-201) and 232 residues from the Fc protein of human IgG1, and has a calculated molecular mass of 109 .6 kDa.
Synonyms: TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, TR1, MGC29565.
Formulation: OPG was lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 6.0, 150 mM NaCl and 0.02 % Tween-80.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Pichia Pastoris.
Amino acid sequence: OPG 22-201 ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQKCGIDVTL Fc232 EPKSSDKTHT CPPCPAPEFE GAPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPTPIEKTISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
Uniprot ACC number: O00300
Transportation method: Shipped at room temperature.
Stability: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by neutralizing the stimulation of U937 cells is less than 10 ng/ml, corresponding to a specific activity of > 100,000 IU/mg in the presence of 10 ng/ml soluble human RANKL (sRANKL).
Solubility: It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com