Recombinant Human Osteoprotegerin produced in
E. coli cells is a single, non-glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of 20 kDa. The OPG is purified by proprietary chromatographic techniques.
Synonyms: TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TR1, MGC29565.
Formulation: The OPG was lyophilized from a 0.2 µm filtered concentrated (0.5 mg/ml) solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK.
Uniprot ACC number: O00300
Transportation method: Shipped at room temperature.
Stability: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The activity is determined by its ability to neutralize the stimulation of U937 cells treated with 10 ng/ml of soluble RANKL corresponding to a specific activity of 100,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com