Recombinant human Secreted pH ospH oprotein-1 produced in
E. coli is single, a non-glycosylated, Polypeptide chain containing 321 amino acids fragment (17-314) and having a total molecular mass of 36.2 kDa(molecular weight on SDS-PAGE will shift up). The SPP1 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms: Secreted pH ospH oprotein-1, OPN, BNSP, BSPI, ETA-1, MGC1109 40, SPP-1, Osteopontin, Bone sialoprotein 1, Urinary stone protein, NepH ropontin, Uropontin, SPP1.
Formulation: Osteopontin is supplied in 20 mM Tris-HCl buffer pH 7.5, 1 mM DTT, 2 mM EDTA and 10% glycerol.
Physical Appearance: Sterile Filtered clear colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKandE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N.
Uniprot ACC number: P10451
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at info@3hbiomedical.com