Recombinant human Neuritin-1 produced in
E. coli is a single, non-glycosylated, polypeptide chain (Ala28-Gly116) containing 99 amino acids including a 10 aa His-Tag at N-terminus. The total calculated molecular mass is 11 kDa.
Synonyms: Neuritin 1, NRN1, NRN, dJ380B8.2, Neuritin.
Formulation: NRN1 was filtered (0.4 µm) and Lyophilized in 20 mM Tris buffer, 50 mM NaCl, pH 8.0, 1% (w/v) Sucrose and 4% (w/v) Mannitol.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHASAGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGNG.
Uniprot ACC number: Q9NPD7
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time.
Solubility: It is recommended to add 200µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. NRN1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com