Recombinant human NOV produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a. 33-357) containing 331 amino acids including a 6 a.a. C-terminal His-Tag. The total molecular mass is 36.5 kDa (calculated).
Synonyms: Protein NOV homolog, NovH, CCN family member 3, nsulin-like growth factor-binding protein 9, IBP-9, IGF-binding protein 9, IGFBP-9, Nephroblastoma-overexpressed gene protein homolog, NOV, CCN3, IGFBP9, NOVH.
Formulation: NOV filtered (0.4 µm) and lyophilized from 0.5 mg/ml in PBS and 5 % (w/v) trehalose.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Source: HEK293 cells.
Amino acid sequence: QRCPPQCPGR CPATPPTCAP GVRAVLDGCS CCLVCARQRG ESCSDLEPCD ESSGLYCDRS ADPSNQTGIC TAVEGDNCVF DGVIYRSGEK FQPSCKFQCT CRDGQIGCVP RCQLDVLLPE PNCPAPRKVE VPGECCEKWI CGPDEEDSLG GLTLAAYRPE ATLGVEVSDS SVNCIEQTTE WTACSKSCGM GFSTRVTNRN RQCEMLKQTR LCMVRPCEQE PEQPTDKKGK KCLRTKKSLK AIHLQFKNCT SLHTYKPRFC GVCSDGRCCT pH NTKTIQAE FQCSPGQIVK KPVMVIGTCT CHTNCPKNNE AFLQELELKT TRGKMHHHHH H.
Uniprot ACC number: P48745
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after one week at +4°C.
Solubility: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. NOV is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com