Recombinant human Total 152AA. M. W.16.7 kDa (calculated). N-terminal His-tag and spacer (43AA – highlighted). The AA sequence of the human myostatin part of the fusion protein is corresponding to the UniProtKB/Swiss-Prot entry O14793.
Synonyms: GDF-8, MSTN, Growth/Differentiation Factor 8, MSTN Muscle HypertropH y.
Formulation: Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.05M acetate buffer, pH 4.5.
Physical Appearance: Filtered white lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSRSAVR SRRDFGLDCD EHSTESRCCR YPLTVDFEAFGWDWIIAPKR YKANYCSGEC EFVFLQKYpH THLVHQANPR GSAGPCCTPT KMSPINMLYF NGKEQIIYGKIPAMVVDRCG CS.
Uniprot ACC number: O14793
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: Add 0.1M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/mL and let the Lyophilized pellet dissolve completely. For conversion into higher pH value, we reco mMend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.
Safety data sheet: Inquire at info@3hbiomedical.com