Recombinant human Midkine produced in
E. coli is a single, non-glycosylated polypeptide chain containing 123 amino acids and having a molecular mass of 13.4 kDa. The Midkine is purified by proprietary chromatographic techniques.
Synonyms: NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in 1×PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD.
Uniprot ACC number: P21741
Transportation method: Shipped at room temperature.
Stability: Lyophilized Midkine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract human neutropH ils using a concentration range of 0.1-10 ng/ml corresponding to a specific activity of 100,000-10,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Midkine in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com