Recombinant human Interleukin-7 produced in yeast is a single, glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.
Synonyms: Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Formulation: lyophilized from a concentrated (1mg/ml) solution in water containing 20 mM phosphate buffer.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by SDS-PAGE.
Source: Saccharomyces cerevisiae.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu.
Uniprot ACC number: P13232
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is < 0.5 ng/ml
, corresponding to a specific activity of > 2 x 106 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com