Recombinant human Interleukin-7 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.
Synonyms: Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Formulation: lyophilized from a concentrated (1mg/ml) solution in water containing 1x PBS pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: DCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH.
Uniprot ACC number: P13232
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.5 ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title: Transfer of mRNA encoding recombinant i mMunoreceptors reprograms CD4+ and CD8+ T cells for use in the adoptive i mMunotherapy of cancerPublication: Gene therapy 16.5 (2009): 596-604. Link: http://www. nature. com/gt/journal/v16/n5/full/gt2008189a. html
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