Recombinant human Interleukin-6 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21 kDa. The IL6 is purified by proprietary chromatographic techniques.
Synonyms: B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Formulation: lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
Uniprot ACC number: P05231
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml
, corresponding to the specific activity of 1.0 x 10,000,000 Units per mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Identification of ?-Escin as a Novel Inhibitor of Signal Transducer and Activator of Transcription 3/Janus-Activated Kinase 2 Signaling Pathway that Suppresses Proliferation and Induces Apoptosis in Human Hepatocellular Carcinoma Cells . Publication: Published online before print April 8, 2010, doi: 10.1124/jpet.110.165498 JPET July 2010 vol.334 no.1 285-293 Link:http://jpet. aspetjournals. org/content/334/1/285. full2. Title:Suppression of Signal Transducer and Activator of Transcription 3 Activation by Butein Inhibits Growth of Human Hepatocellular Carcinoma
in vivo. Publication:Published OnlineFirst December 3, 2010; doi: 10.1158/107 8-0432. CCR-10-1123 Clin Cancer Res March 15, 2011 17; 1425. Link:http://clincancerres. aacrjournals. org/content/17/6/1425. full3. Title:Identification of ?-escin as a novel inhibitor of STAT3/JAK2 signaling pathway that suppresses proliferation and induces apoptosis in human hepatocellular carcinoma cells. Publication:Published online before print April 8, 2010, doi: 10.1124/jpet.110.165498 JPET April 8, 2010 jpet.110.165498Link:http://jpet. aspetjournals. org/content/early/2010/04/09/jpet.110.165498. short4. Title:enhanced self -renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene sall4. Publication:Link:http://www. biomedcentral. com/content/pdf/1756-8722-4-38. pdf5. Title:Thymoquinone inhibits proliferation, induces apoptosis and chemosensitizes human multiple myeloma cells through suppression of signal transducer and activator of transcription 3 activation pathway. Publication:Article first published online: 10 MAY 2010DOI: 10.1111/j.1476-5381.2010.00874. xLink:http://onlinelibrary. wiley. com/doi/10.1111/j.1476-5381.2010.00874. x/full
Safety data sheet: Inquire at info@3hbiomedical.com