Recombinant human Interleukin-4 produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids. The IL-4 is purified by proprietary chromatographic techniques.
Synonyms: BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Formulation: The protein was lyophilized from 0.2 µm filtered solution in 20 mM sodium phosphate buffer pH 6.0 in absence of any carrier protein.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by SDS-PAGE.
Source: Pichia pastoris.
Uniprot ACC number: P05112
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin-4 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The biological activity is determined by measuring the dose-dependent proliferation of human TF–1 cells and CD23 expression. A concentration range of 0.1–10.0 ng/ml is effective for most
in vitro applications. ED50 = 0.05–0.4 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com