Recombinant human Interleukin-4 produced in CHO is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14 kDa, the glycosilation of IL-4 migrates as 18 kDa on SDS-PAGE. The IL-4 is purified by proprietary chromatographic techniques.
Synonyms: BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Formulation: lyophilized from a phosphate- Buffered Saline (pH 7.4).
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Chinese Hamster Ovarian Cells.
Uniprot ACC number: P05112
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2 ng/ml corresponding to a Specific Activity of 5,000,000-20,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title:A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic DiseasePublication:doi: 10.4049/ji mMunol.0804125 The Journal of Immunology May 15, 2009 vol.182 no.10 6011-6021 Link:http://www. ji mMunol. org/content/182/10/6011. full
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