Recombinant human Interleukin-4 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15 kDa. The IL-4 is purified by proprietary chromatographic techniques.
Synonyms: BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Formulation: lyophilized from a 0.2 µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS.
Uniprot ACC number: P05112
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation1Publication:The Journal of Immunology, 2006, 177: 6695-6704. Copyright © 2006 by The American Association of I mMunologists, Inc. Link:http://www. ji mMunol. org/content/177/10/6695. full2. Title:CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the i mMunological synapsePublication:Published online before print June 27, 2005, doi: 10.107 3/pnas.050105 0102 PNAS July 5, 2005 vol.102 no.27 9583-9588 Link:http://www. pnas. org/content/102 /27/9583. full3. Title:A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic DiseasePublication:doi: 10.4049/?ji mMunol.0804125 The Journal of Immunology May 15, 2009 vol.182 no.10 6011-6021 Link:http://www. ji mMunol. org/content/182/10/6011. full4. Title:Defective IL-10 production in severe pH enotypes of Crohn’s diseasePublication:Published online before print February 23, 2009, doi: 10.1189/jlb.1108 698 May 2009 Journal of Leukocyte Biology vol.85 no.5 896-903 Link:http://www. jleukbio. org/content/85/5/896. full5. Title:Adenosine deaminase potentiates the generation of effector, memory, and regulatory CD4+ T cells . Publication:Published online before print October 19, 2010, doi: 10.1189/jlb.1009696 January 2011 Journal of Leukocyte Biology vol.89 no.1 127-136 . Link:http://www. jleukbio. org/content/89/1/127. full6. Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation/Publication:Link:http://journal.9med. net/qikan/article. pH p?id=367793
Safety data sheet: Inquire at info@3hbiomedical.com