Recombinant human IL36G (152 a.a.) produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.0 kDa. The IL36G (152 a.a.) is purified by proprietary chromatographic techniques.
Synonyms: Interleukin 36 gamma, IL1F9, interleukin 1 family member 9, Interleukin-1 epsilon, IL-1RP2, IL-1H1, IL1E, interleukin 1-related protein 2, Interleukin-1 homolog 1.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in 1×PBS, pH 7.4, containing 5% trehalose.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND
Uniprot ACC number: Q9NZH8
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL36g Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36g should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Fully biologically active when compared to standard. The ED50 as measured by its ability to induce IL-8 secretion in human preadipocytes is less than 15 ng/ml, corresponding to a Specific Activity of 67,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IL36g in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com