Recombinant human IL36B 153 a.a.. produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (5-157 a.a.) and having a molecular mass of 17.2 kDa. The IL36B 153 a.a. Human is purified by proprietary chromatographic techniques.
Synonyms: Interleukin 36 beta, interleukin 1 family member 8 (eta), Interleukin-1 homolog 2, IL1F8 (Canonical product IL-1F8a), IL-1F8 (FIL1-eta), Interleukin-1 Superfamily e, IL1H2, MGC126880, MGC126882.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by SDS-PAGE and HPLC analyses.
Source:Escherichia coli. Amino acid sequence: REAAPKSYAI RDSRQMVWVL SGNSLIAAPL SRSIKPVTLH LIACRDTEFS DKEKGNMVYL GIKGKDLCLF CAEIQGKPTL QLKEKNIMDL YVEKKAQKPF LFFHNKEGST SVFQSVSYPG WFIATSTTSG QPIFLTKERG ITNNTNFYLD SVE
Uniprot ACC number: Q9NZH7
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL36B 153 a.a.. Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36B 153 a.a.. Human should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Fully biologically active when compared to standard. The ED50 as measured by its ability to induce IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of 10IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IL36B 153 a.a.. Human in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com