Recombinant human IL36A 158 a.a.. produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.7 kDa. The IL36A 158 a.a. Human is purified by proprietary chromatographic techniques.
Synonyms: Interleukin 36 Alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553.
Formulation: lyophilized from a 0.2?m filtered concentrated solution in 2xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE and HPLC analyses.
Source:Escherichia coli. Amino acid sequence: MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF
Uniprot ACC number: Q9UHA7
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL36A 158 a.a.. Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36A 158 a.a.. Human should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. I mMobilized rHuIL-36? at 1 ?g/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5?g/ml corresponding to a specific activity of 200,000-6, 666, 667IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IL36A 158 a.a.. Human in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com