Recombinant human IL36A produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (aa 6-158) and having a molecular mass of 17.0 kDa. The IL36A is purified by proprietary chromatographic techniques.
Synonyms: Interleukin 36 Alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 8.0.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF
Uniprot ACC number: Q9UHA7
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL36A Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36A should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Fully biologically active when compared to standard. The ED50 as measured by its ability to induce IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a Specific Activity of 100,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IL36A in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com