Recombinant human Interleukin-32 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 131 amino acids and having a molecular mass of 14.9 kDa.
Synonyms: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32Alpha, IL-32delta, IL-32gamma, Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor Alpha-inducing factor, IL-32a, IL32a, IL32, Interleukin-32 Alpha.
Formulation: IL-32 was lyophilized from a concentrated (1mg/ml) solution in water containing 50 mM sodium phosphate buffer pH 7.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K.
Uniprot ACC number: P24001
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL32 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL32 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Human IL-32 Alpha activity is measured via the dose-dependent induction of TNF-Alpha in the human THP-1 monocytic cell line.
Solubility: It is recommended to reconstitute the lyophilized IL-32 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com