Recombinant human IL-31 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids (24-164 a.a.) and having a molecular mass of 15.8 kDa.
Synonyms: Interleukin 31, IL31, IL-31.
Formulation: The IL-31 (1mg/ml) was Lyophilized with 20 mM phosphate buffer, 150 mM NaCl pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T.
Uniprot ACC number: Q6EBC2
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL-31 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-31 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 was determined by its ability to activate STAT following receptor ligand interaction and found to be <5 ng/ml, corresponding to a specific activity of 200,000 units.
Solubility: It is recommended to reconstitute the lyophilized IL-31 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com