Recombinant human IL21 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a total molecular mass of 15.4 kDa. The IL-21 is purified by proprietary chromatographic techniques.
Synonyms: Za11, IL-21.
Formulation: lyophilized from PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS.
Uniprot ACC number: Q9HBE4
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL21 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Measured by its ability to proliferate activated B cells.
Solubility: It is recommended to reconstitute the lyophilized Interleukin -21 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com