Recombinant human Interleukin-17E produced in
E. coli is a non-glycosylated disulfide-linked homodimer containing 2x145 amino acids (with an N-terminal Methionine for each chain total aa for each chain 146) and having a total predicted molecular mass of 33.7 kDa. The IL-25 Human is purified by proprietary chromatographic techniques.
Synonyms: IL-25, IL-17E, IL17E, IL25, Interleukin-25, Interleukin-17E.
Formulation: lyophilized from a concentrated (1mg/ml) solution containing no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CpH CVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG.
Uniprot ACC number: Q9H293
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin Human IL17E although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human IL-25 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent production of IL-8 by Human PBMCs is 26-40 ng/ml, corresponding to a specific activity of 3.8x104 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin Human IL25 in sterile 10 mM HCl not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com