Recombinant human Interleukin-17A produced in
E. coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids (2 chains of 132 aa) and having a molecular mass of 31 kDa. The IL-17 is purified by proprietary chromatographic techniques.
Synonyms: CTLA-8, IL-17, IL-17A, Cytotoxic T-Lymphocyte-associated antigen 8.
Formulation: Sterile filtered lyophilized (freeze-dried) powder, with 20 mM Citrate, 0.1M NaCl, pH 4.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Uniprot ACC number: Q16552
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin 17A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL17 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2 ng/ml corresponding to a Specific Activity of 500,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 17 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com