Recombinant human Interleukin-16 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 13.5 kDa. The IL-16 is purified by proprietary chromatographic techniques.
Synonyms: IL16, Interleukin-16, LCF, Lymphocyte Chemoattractant Factor, prIL-16, IL-16, FLJ16806, FLJ42735, FLJ44234, HsT19289.
Formulation: IL-16 was Lyophilized at 1 mg/ml in 10 mM NaP, pH 7.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by: (a) Analysis by RP-HPLC. (b) Analysis SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Asp-Leu-Asn.
Uniprot ACC number: Q14005
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-16 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL16 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by its ability to chemoattract human CD4+ T Lymphocytes using a concentration range of 10-100 ng/ml, corresponding to a Specific Activity of 10,000-100,000 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-16 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com