Recombinant human Interleukin-15 His ?produced in
E. coli is single, a non-glycosylated, Polypeptide chain containing 123 amino acids fragment (49-162) having a total molecular weight of 13.9 kDa with an C-terminal hexahistidine tag. The IL-15 His is purified by proprietary chromatographic techniques.
Synonyms: IL-15, MGC9721.
Formulation: IL-15 His is supplied in 20 mM Tris-HCl buffer (pH 8.0) 0.2 mM PMSF and 10% glycerol.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH.
Uniprot ACC number: P40933
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Biological Activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2.5 ng/ml, corresponding to a Specific Activity of 400,000units/mg.
Safety data sheet: Inquire at info@3hbiomedical.com