Recombinant human Interleukin-13 Variant produced in
E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13, having a molecular mass of 12.5 kDa. The IL-13 Variant is purified by proprietary chromatographic techniques.
Synonyms: Interleukin-13, NC30, ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.2, containing 5% trehalose.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCpH KVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GQFN.
Uniprot ACC number: P35225
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-13 Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 Variant should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be 1,000,000 units/mg. This analog has also been shown to exhibit increased
in vivo activity compared to wild type IL-13.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 13 Variant in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com