Recombinant human IL12 produced in a baculovirus expression system is a glycosylated disulfide linked (through cysteines in bold) heterodimer comprised of IL12A (23-219aa, total of 203 aa, MW 23.3 kDa) and IL12B (23-328aa, total of 306 aa, MW 34.6 kDa), having a total predicted molecular mass of 57.9 kDa (Molecular weight on SDS-PAGE will appear higher). IL12A is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Synonyms: 23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 12 (subunit beta/Alpha), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic Lymphocyte maturation factor (CLMF), TSF, Edodekin-Alpha, IL-12.
Formulation: IL12 protein solution (0.5 mg/ml) containing phosphate buffered saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source: Baculovirus.
Amino acid sequence: IL12A(p35):RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSF mMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH. IL12B(p40):IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STpH SYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS.
Uniprot ACC number:Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml.
Safety data sheet: Inquire at info@3hbiomedical.com