Recombinant human Interleukin 12 is a glycosylated heterodimer containing 503 amino acids (306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits) and having a molecular mass of 60 kDa. IL12 is purified by proprietary chromatographic techniques.
Synonyms: 23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 12 (subunit beta/Alpha), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic Lymphocyte maturation factor (CLMF), TSF, Edodekin-Alpha, IL-12.
Formulation: lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by analysis by SDS-PAGE.
Source: CHO Cells.
Amino acid sequence: IL-12 is a heterodimer of IL-12A and IL-12B linked through a disulfide-bond: >IL-12 ARNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSF mM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS. >IL-12B IWELKKDV YVVELDWYPD APGEMVVLTC DTPEEDGITW TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS LLLLHKKEDG IWSTDILKDQ KEPKNKTFLR CEAKNYSGRF TCWWLTTIST DLTFSVKSSR GSSDPQGVTC GAATLSAERV RGDNKEYEYS VECQEDSACP AAEESLPIEV MVDAVHKLKY ENYTSSFFIR DIIKPDPPKN LQLKPLKNSR QVEVSWEYPD TWSTpH SYFS LTFCVQVQGK SKREKKDRVF TDKTSATVIC RKNASISVRA QDRYYSSSWS EWASVPCS.
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin 12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Determined by cell proliferation assay using pH A-stimulated human T lympH oblasts. The ED50 for this effect is 0.01-0.05 ng/ml corresponding to a specific activity of 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com