Recombinant human Interleukin-10 produced in HEK cells is a glycosylated non-disulfide bonded homodimer, having a total molecular weight of 17 kDa. The IL10 is purified by proprietary chromatographic techniques.
Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Formulation: The IL10 was lyophilized from 1mg/ml in 10 mM Tris-HCl, pH 7.4 and 150 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Uniprot ACC number: P22301
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/ml IL-4, and is typically 0.3-1.5 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized IL-10 in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com