Recombinant human IL1ra produced in
E. coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17 kDa. The IL1ra is purified by proprietary chromatographic techniques.
Synonyms: IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, MGC10430.
Formulation: Lyophilized protein with no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.
Uniprot ACC number: P18510
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin 1ra although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1ra should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml
corresponding to a Specific Activity of 2,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-1ra in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title:Interleukin 1 receptor antagonist mediates the antiInflammatory and antifibrotic effect of mesenchymal stem cells during lung injury. Publication:Published online before print June 14, 2007, doi: 10.107 3/pnas.0704421104 PNAS June 26, 2007 vol.104 no.26 11002-11007Link:http://www. pnas. org/content/104/26/11002. short
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