Recombinant human Interleukin-1 beta produced in
E. coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17 kDa. The IL-1b is purified by proprietary chromatographic techniques.
Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 50 mM phosphate buffer pH 7.1 and 150 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser.
Uniprot ACC number: P01584
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in the test of augmentation of Lymphocyte proliferation assay using mouse thymus was found to be 200,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-1b in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title: ProInflammatory Cytokine IL-1b Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis. Publication: Published online before print March 1, 2010, doi: 10.4049/ ji mMunol.0901735 The Journal of Immunology April 1, 2010 vol.184 no.7 3946-3954. Link: http://www. ji mMunol. org/content/184/7/3946. fullApplication: Level of IL-8 mRNA was dramatically enhanced by IL-1b, HMC-1 cells were stimulated with IL-1b. 2. Title:Contribution of IL-1 to resistance to Streptococcus pneumoniae infection . Publication: Int. I mMunol. (2008) 20 (9): 1139-1146. doi: 10.109 3/inti mM/dxn071 First published online: July 1, 2008 Link:http://inti mM. oxfordjournals. org/content/20/9/1139. full3. Title: Hydrogen sulpH ide decreases IL-1b induced activation of fibroblast?like synoviocytes from patients with osteoarthritisPublications: journal of cellular and molecular medicine 19.1 (2015): 187-197Link: http://onlinelibrary. wiley. com/doi/10.1111/jc mM.12405/epdf 4. Title: Riampin Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial CellsPublications: Antimicrobial agents and chemotherapy 50.1 (2006): 396-398Link: http://aac. asm. org/content/50/1/396. full 5. Title: Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proInflammatory mediatorsPublication: Arthritis Res Ther 11.6 (2009): R161. Link: http://www. biomedcentral. com/content/pdf/ar2844. pdf
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