Recombinant human IFNG produced in
E. coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (24-161 a.a.) and having a molecular mass of 18.4 kDa. IFNG is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Formulation: IFNG protein solution (1mg/ml) containing phosphate buffered saline (pH 7.4) and 20% glycerol.
Physical Appearance: Sterile Filtered colorless liquid.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG.
Uniprot ACC number: P01579
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is ≤ 5 ng/ml.
Safety data sheet: Inquire at info@3hbiomedical.com