Recombinant human Interferon Alpha Human 2a Recombinant produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19 kDa. The Interferon-a 2a contains affinity 6xHis-Tag on C-terminus. The IFN-A 2a is purified by proprietary chromatographic techniques.
Synonyms: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.
Formulation: Lyophilized, containing 50% SDS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 99.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Nicotiana Sp. Plant.
Amino acid sequence: The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.
Uniprot ACC number: P01563
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interferon Alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2a should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 × 108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Solubility: It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com