Recombinant human Interferon-alpha 1b produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19.4 kDa. The Interferon-alpha 1b gene was obtained from human leukocytes. The IFN-a 1b is purified by proprietary chromatographic techniques.
Formulation: lyophilized from a 0.2 µm filtered solution (50ug/ml) in PBS, pH 7.4, containing 4% mannitol and 1% HSA.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.
Uniprot ACC number: Q8WZ68
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interferon Alpha 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 1b should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 108 IU/ mg.
Solubility: It is recommended to reconstitute the lyophilized IFN Alpha 1b in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com