Recombinant human Insulin Like Growth Factor Binding Protein-4 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 237 amino acids and having a molecular mass of 30 kDa. The IGFBP4 is purified by proprietary chromatographic techniques.
Synonyms: Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP.
Formulation: lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0 and 150 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Sf9, Insect cells.
Amino acid sequence: DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE.
Uniprot ACC number: P22692
Transportation method: Shipped at room temperature.
Stability: Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin Like Growth Factor Binding Protein-4 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 is determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells and is <than 0.1 μg/ml, corresponding to a specific activity of > 1.0 × 104 IU/mg in the presence of 14 ng/ml
of rHuIGF-II.
Solubility: It is recommended to reconstitute the lyophilized Insulin Like Growth Factor Binding Protein-4 in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com