Recombinant human IGFBP3 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (28-291 a.a.) and having a molecular mass of 31 kDa. IGFBP3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: Insulin-like growth factor-binding protein 3, Insulin Like Growth Factor Binding Protein-3, His-Tag, IGFBP3, IBP-3, IGF-binding protein 3, IGFBP-3, IBP3, BP-53, Insulin-like growth factor binding protein 3 isoform b precursor.
Formulation: IGFBP3 protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 90% as determined by analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGASSAGLGP VVRCEPCDAR ALAQCAPPPA VCAELVREPG CGCCLTCALS EGQPCGIYTE RCGSGLRCQP SPDEARPLQA LLDGRGLCVN ASAVSRLRAY LLPAPPAPGN ASESEEDRSA GSVESPSVSS THRVSDPKFH PLHSKIIIIK KGHAKDSQRY KVDYESQSTD TQNFSSESKR ETEYGPCRRE MEDTLNHLKF LNVLSPRGVH IPNCDKKGFY KKKQCRPSKG RKRGFCWCVD KYGQPLPGYT TKGKEDVHCY SMQSK.
Uniprot ACC number: P17936
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at info@3hbiomedical.com