Recombinant human Insulin produced in
E. coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.8 kDa. Insulin is purified by proprietary chromatographic techniques.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by RP-HPLC analysis.
Source:Escherichia coli. Uniprot ACC number: P01308
Transportation method: Shipped at room temperature.
Stability: Lyophilized Insulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The Biological Activity was determined to be 28 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Insulin in sterile 0.005N HCl not more than 1 mg/ml.
References: Title: PI3K integrates the effects of insulin and leptin on large-conductance Ca2+-activated K+ channels in neuropeptide Y neurons of the hypothalamic arcuate nucleusPublication: American Journal of pH ysiology-Endocrinology and Metabolism 298.2 (2010): E193-E201. Link: http://ajpendo. pH ysiology. org/content/298/2/E193. shorty
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