Recombinant human IFN-Omega 1 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19.9 kDa. The IFN-Omega 1 is purified by proprietary chromatographic techniques.
Synonyms: IFN omega-1, IFN Alpha-II-1, IFNW1.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MCDLPQNHGL LSRNTLVLLH QMRRISPFLC LKDRRDFRFP QEMVKGSQLQ KAHVMSVLHE MLQQIFSLFH TERSSAAWNM TLLDQLHTGL HQQLQHLETC LLQVVGEGES AGAISSPALT LRRYFQGIRV YLKEKKYSDC AWEVVRMEIM KSLFLSTNMQ ERLRSKDRDL GSS.
Uniprot ACC number: P05000
Transportation method: Shipped at room temperature.
Stability: Lyophilized IFNW1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNW1 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 was determined by a cytotoxicity assay using Human TF-1 cells is < 0.01 ng/ml
, corresponding to a specific activity of > 100,000,000 units/mg.
Solubility: It is recommended to reconstitute the lyophilized IFNW1 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com