Recombinant human IFN-beta 1a produced in CHO (Chinese Hamster Ovarian) cells is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22.5 kDa. IFN-beta1a is purified by proprietary chromatographic techniques.
Synonyms: Leukocyte IFN, B cell IFN, Type I IFN, IFNB1, IFB, IFF, IFNB, IFN-b 1a , MGC96956.
Formulation: lyophilized from a solution containing 91 mg Human Albumin, and 620 mg mannitol and 50 mM Acetate acid pH 3.8.
Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: CHO (Chinese Hamster Ovarian) cells.
Uniprot ACC number: P01574
Transportation method: Shipped at room temperature.
Stability: Lyophilized IFN-beta 1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Beta1a should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 106 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IFN beta 1a in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: Title:IFNG induces apoptosis in human SH-SY5Y neuroblastoma cells through activation of JAK–STAT signaling and down-regulation of PI3K/Akt pathway. Publication:Article first published online: 11 NOV 2010 DOI:10.1111/j.1471-4159.2010.07046. xLink:http://onlinelibrary. wiley. com/doi/10.1111/j.1471-4159.2010.07046. x/full
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