Recombinant human IFN Alpha Human 2a Recombinant produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19.2 kDa The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine. The IFNA2A gene was obtained from human leukocytes. The IFNA2A is purified by proprietary chromatographic techniques.
Synonyms: Leukocyte IFN, B cell IFN, Type I IFN, IFNA2, IFN-a 2a.
Formulation: Lyophilized without additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEM IQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAV RKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE. N-terminal methionine has been completely removed enzymatically.
Uniprot ACC number: P01563
Transportation method: Shipped at room temperature.
Stability: Lyophilized IFNA-2A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2a should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IFNA2A in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Gender specificity of altered human Immune cytokine profiles in aging. Publication:Published online before print May 7, 2010, doi: 10.109 6/fj.10-160911 September 2010 The FASEB Journal vol.24 no.9 3580-3589 Link:http://www. fasebj. org/content/24/9/3580. full 2. Title: Differential involvement of 5-HT1A and 5-HT1B/1D receptor in human IFN a-induced i mMobilityPublication: Arzneimittel-Forschung 60.3 (2009): 109 -115. Link: http://www. ijppp. org/files/IJPPP905002. pdf
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