Recombinant human HB-EGF produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 87 amino acids and having a molecular mass of 9.9 kDa. The HB-EGF is purified by proprietary chromatographic techniques.
Synonyms: HBEGF, DTR, DTS, HEGFL, HB-EGF, DipH theria toxin receptor, DT-R, DTSF.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution containing 10 mM sodium phosphate pH 7.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MDLQEADLDL LRVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK YKDFCIHGEC KYVKELRAPS CICHPGYHGE RCHGLSL.
Uniprot ACC number: Q99075
Transportation method: Shipped at room temperature.
Stability: Lyophilized Human HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 was determined by the ability to induce proliferation of 3T3 cells and is 0.13-0.2 ng/ml. This corresponds to an expected specific activity of 7.7 x 106 units/mg.
Solubility: It is recommended to reconstitute the lyophilized Human HB-EGF in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com