Recombinant human Growth Hormone Placental 20 kDa produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20.5 kDa. Predicted pI 8.20. Growth Hormone 20K placental is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs. GH 20K placental is purified by proprietary chromatographic techniques.
Synonyms: GHL, GHV, GH-V, hGH-V, PGH.
Formulation: pGH 20kDa was lyophilized from a concentrated (1mg/ml) solution with0.0045 mM NaHCO3 previously adjusted pH 11.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: AFPTI PLSRLFDNAM LRARRLYQLA YDTYQEFNPQ TSLCFSESIP TPSNRVKTQQ KSNLELLRIS LLLIQSWLEP VQLLRSVFAN SLVYGASDSN VYRHLKDLEE GIQTLMWRLE DGSPRTGQIF NQSYSKFDTK SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF
Uniprot ACC number: O14643
Transportation method: Shipped at room temperature.
Stability: Lyophilized Growth Hormone 20K Placental although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH 20K pl can be stored at +4°C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized Placental Growth Hormone in 0.4% NaHCO3or water adjusted to pH 11, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com